CAS Chem.name Chem.SMILES #Observed Mutagenicity Bioassay - Reference(s) - 55-18-5 N-Nitrosodiethylamine CCN(CC)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240; P&G Inventory 59-89-2 N-nitrosomorpholine O=NN1CCOCC1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Zeiger, E., Anderson, B., Haworth, S., Lawlor, T. And Mortelmans, K.; Salmonella Mutagenicity Tests. V. Results From The Testing of 311 Chemicals; Environ. Mol. Mutagen. 19(SUPPL.21):2-141, 1992 62-75-9 N-nitrosodimethylamine CN(C)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240; P&G Inventory 100-75-4 N-Nitrosopiperidine O=NN1CCCCC1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240; P&G Inventory 842-07-9 C.I. Solvent Yellow 14 Oc1ccc2ccccc2c1N=Nc1ccccc1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240; P&G Inventory 2646-17-5 Solvent Orange 2 Cc1ccccc1N=Nc1c(O)ccc2ccccc21 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) P&G Inventory 7247-89-4 Piperidine, 2-methyl-N-nitroso- CC1CCCCN1N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240 3817-11-6 n-butyl-n-(4-hydroxybutyl)nitrosamine;4-(butylnitrosoamino)-1-butanol CCCCN(CCCCO)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency, Mutation Research, 168 (1986) 69-240 55557-00-1 1h-1,4-diazepine, hexahydro-1,4-dinitroso- O=NN1CCCN(CC1)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240 140-79-4 dinitrosopiperazine O=NN1CCN(CC1)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240 924-16-3 n,n-dibutylnitrosamine;nitrosodibutylamine CCCCN(CCCC)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) CCRIS database,Toxnet databases: Rumruen, K. And Pool, Bl.; Metabolic Activation Capabilities Of S9 And Hepatocytes From Uninduced Rats To Convert Carcinogenic N-Nitrosamines To Mutagens; Mutat. Res. 140(2-3):147-153, 1984 89911-79-5 n-nitroso-2,3-dihydroxypropyl-2-hydroxypropylamine CC(O)CN(CC(O)CO)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) MORI,Y, YAMAZAKI,H, TOYOSHI,K, DENDA,A AND KONISHI,Y; MUTAGENIC ACTIVATION OF CARCINOGENIC N-NITROSOPROPYLAMINES BY LIVER S9 FRACTIONS FROM MICE, RATS AND HAMSTERS. EVIDENCE FOR A CYTOCHROME P-450-DEPENDENT REACTION; CARCINOGENESIS 7(3):375-379, 1986 621-64-7 N,N-dipropylnitrosamine CCCN(CCC)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Dahl ,Ar.; Mutagenicity Of Some Dialkylnitrosamines, Cyclic Nitrosamines And N,N-Diethanolnitrosamine In Salmonella Typhimurium With Rat And Rabbit Nasal, Lung And Liver S9 Homogenates; Mutat. Res. 158(3):141-147, 1985 937-40-6 n-methyl-n-benzylnitrosamine CN(Cc1ccccc1)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) SINGER,GM, ANDREWS,AW AND GUO,SM; QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP OF THE MUTAGENICITY OF SUBSTITUTED N-NITROSO-N-BENZYLMETHYLAMINES: POSSIBLE IMPLICATIONS FOR CARCINOGENICITY; J. MED. CHEM. 29(1):40-44, 1986 16543-55-8 (S)-N'-Nitrosonornicotine O=NN1CCCC1c1cccnc1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Padma, P.R., Amonkar, A.J., Bhide, S.V., Mutagenic and Cytogenetic Studies of N'-Nitrosonornicotine and 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone. Cancer Lett., 1989, 46(3), 173 - 180 5632-47-3 N-nitrosopiperazine O=NN1CCNCC1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Zeiger E, Legator MS, Lijinsky W. Mutagenicity of N-nitrosopiperazines for Salmonella typhimurium in the host-mediated assay. Cancer Res. 1972 Jul;32(7):1598-9. 930-55-2 N-nitrosopyrrolidine O=NN1CCCC1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) CCRIS database,Toxnet databases: Guttenplan, J. B., N-Nitrosamines: Bacterial Mutagenesis and In Vitro Metabolism, Mutat. Res. 186 (1987), 81 – 134 64091-91-4 4-(n-methyl-n-nitrosamino)-1-(3-pyridyl)-1-butanone CN(CCCC(=O)c1cccnc1)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) CCRIS database,Toxnet databases: Zhu, S., Cunningham, Ml., Gray, Te. And Nettesheim, P.; Cytotoxicity, Genotoxicity And Transforming Activity Of 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone (Nnk) In Rat Tracheal Epithelial Cells; Mutat. Res. 261(4):249-259, 1991 1116-54-7 N-Nitrosodiethanolamine OCCN(CCO)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) National Toxicology program. P&G Inventory 16048-37-6 1-Anthracenediazonium, 9,10-dihydro-9,10-dioxo-, chloride [Cl-].O=C1c2ccccc2C(=O)c2c(cccc12)[N+]#N in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Brown, J. P. and Brown, R.J.; Mutat. Res. 40:203-224, 1976 122341-55-3 Diazo-N-nitrosoetilefrin CCN(CC(O)C1C=CC(=[N+]=[N-])C(=O)C=1)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Kikugawa, K., Kato, T. and Takeda, Y.; Formation of a Highly Mutagenic Diazo Compound from the Bamethan-Nitrite Reaction; Mutat. Res. 177(1):35-43, 1987 70-25-7 N-Methyl-N'-Nitro-N-Nitrosoguanidine CN(N=O)C(=N)N[N+]([O-])=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Diehl, M. And Fort, F.; Spiral Salmonella Assay: Validation Against The Standard Pour-Plate Assay; Environ. Mol. Mutagen. 27(3):227-236, 1996 456-27-9 Benzenediazonium, 4-nitro-, tetrafluoroborate(1-) F[B-](F)(F)F.[O-][N+](=O)c1ccc(cc1)[N+]#N in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Mersch-Sundermann, V. and Kraemer, H.; The Influence Of Culture Conditions On The Susceptibility Of Salmonella Typhimurium In The Salmonella Mutagenicity Test; Zentralbl. Hyg. Umweltmed. 193(5):471-480, 1993 116539-70-9 2,4-Cyclohexadien-1-one, 4-(2-(butylnitrosamino)-1-hydroxyethyl)-6- CCCCN(C[C@H](O)C1C=CC(=O)C(C=1)=[N+]=[N-])N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Kikugawa, K., Kato, T. and Takeda, Y.; Formation of a Highly Mutagenic Diazo Compound from the Bamethan-Nitrite Reaction; Mutat. Res. 177(1):35-43, 1987 56222-35-6 3-hydroxy-1-nitrosopyrrolidine OC1CCN(C1)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240 55556-92-8 N-Nitroso-1,2,3,6-tetrahydropyridine O=NN1CCC=CC1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) A report of the U.S. Environmental Protection Agency Gene-Tox Program, Mutat. Res. 168:69-240, 1986 7068-83-9 N-Methyl-N-nitroso-1-butanamine CCCCN(C)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency, Mutation Research, 168 (1986) 69-240 61499-28-3 N-Nitroso(2-hydroxypropyl)(2-oxopropyl)amine CC(O)CN(CC(C)=O)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) PubChem database, https://pubchem.ncbi.nlm.nih.gov 614-00-6 N-Methyl-N-nitrosoaniline CN(c1ccccc1)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) PubChem database, https://pubchem.ncbi.nlm.nih.gov/bioassay/1259407#sid=363898350§ion=Test-Results 456-27-9 Benzenediazonium, 4-nitro-, tetrafluoroborate(1-) F[B-](F)(F)F.[O-][N+](=O)c1ccc(cc1)[N+]#N in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Mersch-Sundermann, V. and Kraemer, H.; The Influence Of Culture Conditions On The Susceptibility Of Salmonella Typhimurium In The Salmonella Mutagenicity Test; Zentralbl. Hyg. Umweltmed. 193(5):471-480, 1993 154-93-8 carmustine ClCCNC(=O)N(CCCl)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency, Mutation Research, 168 (1986) 69-240; Kazius J, McGuire R., Bursi R. Journal of Medicinal Chemistry 48 (1), pp. 312-32, 25 116539-70-9 2,4-Cyclohexadien-1-one, 4-(2-(butylnitrosamino)-1-hydroxyethyl)-6- CCCCN(C[C@H](O)C1C=CC(=O)C(C=1)=[N+]=[N-])N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Kikugawa, K., Kato, T. and Takeda, Y.; Formation of a Highly Mutagenic Diazo Compound from the Bamethan-Nitrite Reaction; Mutat. Res. 177(1):35-43, 1987 869-01-2 n-butyl-n-nitrosourea; 1-butyl-1-nitrosourea CCCCN(N=O)C(N)=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) CCRIS database,Toxnet databases: Janzowski, C, Jacob, D, Henn, I, Zankl, H, Pool-Zobel, Bl, Wiessler, M And Eisenbrand, G;Mechanism Of Action Of The Urinary Bladder Carcinogen N-Nitrosobutyl-3-Carboxypropylamine; Iarc Sci. Publ. 105(Relevance Hum. Cancer N-Nitroso Compd., Tob. Mycotoxins):332-338, 1991 13909-09-6 Semustine; 1-(2-Chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea CC1CCC(CC1)NC(=O)N(CCCl)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) CCRIS database,Toxnet databases: Ashby, J, et. el.; Mutagenicity to Salmonella, Drosophila and the Mouse Bone Marrow of the Human Antineoplastic Agent Fotemustine: Prediction Of Carcinogenic Potency; Mutat. Res. 286(1):101- 109, 1993 38434-77-4 ethylnitrosocyanamide CCN(N=O)C#N in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240; CCRIS database,Toxnet databases: Lee, K., B. Gold, S. S. Mirvish, Mutagenicity of 22 N-Nitrosamides and Related Compounds for Salmonella typhimurium TA1535, Mutat. Res. 48 (1977), 131 – 138 759-73-9 1-ethyl-1-nitrosourea CCN(N=O)C(N)=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) CCRIS database,Toxnet databases: Ashby, J., Tinwell, H. and Callander, Rd.; Activity of Urethane and N,N-Dimethylurethane in the Mouse Bone-Marrow Micronucleus Assay: Equivalence of Oral and Intraperitoneal Routes of Exposure; Mutat. Res. 245(3):227-230, 1990 92118-27-9 fotemustine CCOP(=O)(OCC)C(C)NC(=O)N(CCCl)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) CCRIS database,Toxnet databases: Ashby, J, et. el.; Mutagenicity to Salmonella, Drosophila and the Mouse Bone Marrow of the Human Antineoplastic Agent Fotemustine: Prediction Of Carcinogenic Potency; Mutat. Res. 286(1):101- 109, 1993 18774-85-1 n-hexylnitrosourea;urea, 1-hexyl-1-nitroso- CCCCCCN(N=O)C(N)=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240 13743-07-2 1-(2-hydroxyethyl)-1-nitrosourea NC(=O)N(CCO)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240 33868-17-6 methylnitrosocyanamide CN(N=O)C#N in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240 614-95-9 nitrosoethylurethane CCOC(=O)N(CC)N=O in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) U.S. Environmental Protection Agency; Mutation Research, 168 (1986) 69-240 *10006-72-7 4,5-dicyano-2H-imidazole-2-diazonium chloride N#CC1C(C#N)=NC(N{+}(#N).Cl{-})N=1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Division of Genetic and Mutagenesis, National Institute of Health Science, Japan. ANEI-HOU, AMES/ QSAR International Collaborative Study; http://www.nihs.go.jp/dgm/amesqsar.html. Divizion of Genetic and Mutagenesis, National Institute of Health Science, Japan. ANEI-HOU, AMES/ QSAR International Collaborative Study; http://www.nihs.go.jp/dgm/amesqsar.html; Last update; June 2019 158091-65-7 2,4,6-trimethylbenzenediazonium chloride Cc1cc(C)cc(C)c1N{+}(#N).Cl{-} in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Division of Genetic and Mutagenesis, National Institute of Health Science, Japan. ANEI-HOU, AMES/ QSAR International Collaborative Study; http://www.nihs.go.jp/dgm/amesqsar.html. Divizion of Genetic and Mutagenesis, National Institute of Health Science, Japan. ANEI-HOU, AMES/ QSAR International Collaborative Study; http://www.nihs.go.jp/dgm/amesqsar.html; Last update; June 2019 4346-59-2 4-methoxybenzenediazonium chloride COc1ccc(N{+}(#N).Cl{-})cc1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Division of Genetic and Mutagenesis, National Institute of Health Science, Japan. ANEI-HOU, AMES/ QSAR International Collaborative Study; http://www.nihs.go.jp/dgm/amesqsar.html. Divizion of Genetic and Mutagenesis, National Institute of Health Science, Japan. ANEI-HOU, AMES/ QSAR International Collaborative Study; http://www.nihs.go.jp/dgm/amesqsar.html; Last update; June 2019 85622-93-1 3-methyl-4-oxoimidazo[5,1-d][1,2,3,5]tetrazine-8-carboxamide CN1C(=O)N2C=NC(C(N)=O)=C2N=N1 in vitro Ames positive Bacterial Reverse Mutation Assay (e.g. Ames Test) Division of Genetic and Mutagenesis, National Institute of Health Science, Japan. ANEI-HOU, AMES/ QSAR International Collaborative Study; http://www.nihs.go.jp/dgm/amesqsar.html. Divizion of Genetic and Mutagenesis, National Institute of Health Science, Japan. ANEI-HOU, AMES/ QSAR International Collaborative Study; http://www.nihs.go.jp/dgm/amesqsar.html; Last update; June 2019